Practical HPLC Method Development (Relié)

This new edition of the reference maps the future of high-performance liquid chromatography for years to come. Thoroughly revised and greatly expanded, the Second Edition of this landmark work brings scientists completely up to date on method development for HPLC, one of the main analytical techniques in use today. The authors, widely recognized as preeminent authorities in the field, offer the most far-reaching treatment available, taking into account advances in chromatography and the increasingly complex sample types that have become significant in the past decade.
This cutting-edge reference now includes : Comprehensive coverage of all of the essential steps of method development : sample pretreatment, detection of sample bands, choosing separation conditions, quantitation, and method validation and transfer. Increased focus on specialized applications for separating such newly important analytes as biological samples, mixtures of enantiomers, and preparative separations.
A complete, accessible review of HPLC separation fundamentals : the basic process, column efficiency resolution, sample size, detection and selection of column type and mobile phase . New and enhanced discussions of computer-assisted method development, HPLC column technology, and biochemical and chiral applications. A new, more efficient approach for developing reversed-phase HPLC methods. Exhaustive indexing and cross-referencing, enabling quick access to analysis procedures for different kinds of samples.
The result of years of pathbreaking laboratory research, this invaluable reference stands at the forefront of chemical analysis.