HPLC Made to Measure - A Practical Handbook for Optimization (Relié)

Alongside the development of new ones, the optimization of existing methods is a key task in the HPLC laboratory. Nowadays, this task has to be solved increasingly rapidly and cost-efficiently. This handbook provides well-founded assistance in better meeting this challenge. Internationally renowned authors cover both the general basics and strategies in optimization, as well as the specific aspects involved in such different methods as RP-HPLC, NP-HPLC, micro- and nano-HPLC, and hyphenated techniques such as LC/MS.
Topics such as column selection and chiral separations are also covered. Some of the contributions present applications using common optimization software, such as DryLab and ChromSword. All of the chapters concentrate on the essentials and are written in a practically oriented style, and their self-contained structure allows for targeted references. Throughout the text, the authors offer concrete, practical tips as well as pertinent background information, together with insights into the optimization methods of seven major international companies from various sectors.
The whole is rounded off with real-life reports from experienced users active in the different areas of application, in particular the life sciences, such as proteomics and drug development.